THE FUNGUS LINK PDF
Siamo un'Associazione aperta a ragazzi e ragazze che vogliono vivere lo scoutismo cattolico. Il nostro movimento educativo vuole sviluppare il carattere. dinners. Look for it! Note: (1.) Honey could be an occasional exception, since it does have some antifungal properties. Ref. The Fungus Link by Doug Kaufmann . THE FUNGUS LINK. Quality of Foods: If possible eat ONLY ORGANIC while on this diet. Please follow the diet and do NOT deviate from the allowed foods.
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pdf - dnisterz home - get instant access to ebook the fungus link book pdf at our diabetes fungus link series ppti.info the best home remedies for toenail fungus. The Fungus Link [Doug Kaufmann] on ppti.info *FREE* shipping on qualifying offers. Shows some signs of wear, and may have some markings on the. The Fungus Link to Disease Philosophy Phase One and Two of The Kaufmann Diet were designed with an idea in mind: Fungi and yeasts can become parasitic .
Full size image Considering that CreA has been suggested as the regulator mediating the use of preferred sugars first, we also analyzed sugar consumption in a creA deletion mutant. However, this showed that sequential sugar consumption by A. This is not due to the concentration of the sugar as we previously demonstrated that already at concentrations between 1—10 mM, CreA causes repression of several genes 14 , even though the strength of the repressing effect is dependent on the specific sugar Interestingly, in a previous study with A.
However, in addition to the possible differences between A. In the creA mutant maltose was converted to D-glucose slightly faster than in the cultures of the wild type strain, most probably due to high glucoamylase production in the CreA deletion strain The complete depletion of D-fructose 18 h and D-galacturonic acid 33 h was slightly delayed compared to the wild type. CreA regulates the expression of several catabolic pathway genes, such as several genes involved in L-arabinose catabolism 5 , but based on our results this effect does not extend to the overall uptake of the sugars entering these pathways.
This is somewhat surprising, due to several reports of individual sugar transporters being under CreA control 17 , 18 , Our results from the CreA mutant also demonstrate that in contrast to what was proposed in a recent study, where only the A. While we cannot exclude that indirect effects of CreA, e. No visual reduction in growth was observed for the creA mutant, but even if a small reduction occurred, it does not change the fact that the order of sugar uptake is identical to the wild type.
In addition, the acidification in the culture of the creA mutant was only somewhat delayed compared to the wild type, but reached the same level Supplementary Fig.
S1 , suggesting also only a small difference in growth between the strains. It is unlikely that this delayed acidification would hide an effect on sequential uptake of the sugars.
It could be that if cultures of these strains were compared in which direct spore inoculation, rather than transfers were used a larger difference would be detected, as the growth defect of the creA mutant on plate appears to be mainly during germination and early growth stages unpublished data.
However, we chose to use transfers to in fact avoid differences caused by such effects, as this allowed us to initiate the mixed sugar cultivations with the same amount of already growing mycelium. It has been demonstrated in A. Following sugar uptake in cultures starting from spores, would therefore likely be heavily affected by the differences that occur during germination. Previous studies have also clearly demonstrated the value of transfer cultures to identify transcriptional differences between strains and conditions 8 , 13 , 14 , as this ensures the most similar possible starting point at the moment the strains are confronted with the conditions of interest.
In addition, transfer cultures are also commonly used in fungal biotechnology, where strains are pre-grown at smaller scale before being transferred to the larger bioreactors. Therefore, the use of transfer cultures in our experiment also enables an easier transfer of knowledge to biotechnological companies, as it is more similar to their approach.
Transcriptomics reveals temporal expression profiles during cultivation of A. As CreA did not appear to be regulating the uptake, gene expression was only studied in the wild type A.
Little is known about sugar uptake systems in fungi, except for extensive studies in the model yeast Saccharomyces cerevisiae. Sugar transporters play a key role in fungal growth and adaptation to environments with different carbon sources. So far, only 42 A. Distinct sets of putative sugar transporter encoding genes were highest expressed at the first, second and fourth time points as shown by the rank-based imaging left cluster, Fig.
However, most of these genes were expressed at low level right cluster, Fig. The expression patterns of some of the highly expressed sugar transporter encoding genes may explain part of the observed sugar uptake. However, the functional homology-based annotation should be used with caution, as it has previously been shown that sequence similarity does not always guarantee similarity in function, as transporters with different functions group together in a large scale phylogeny of Aspergillus putative sugar transporters 1.
In addition, it is not known whether most of these transporters are high- or low-affinity transporters, which would affect at which time point they would be most useful to the fungus. Figure 2 Clustering of the expression profiles of genes encoding putative sugar transporters.
The data was normalized and expressed as linear values. The expression values were used for Hierarchical Clustering in Genesis 38 using Pearson correlation and complete linkage and visualized using rank based left and traditional right imaging. The table on the right provides the functional and homology based annotation for the putative transporters.
Characterized transporters in A. Functional annotation was added based on experimental data in A. Full size image Cluster B contains two experimentally proven hexose transporters 1 that were mainly expressed from 18 h onwards.
One of these can only transport D-galactose An06g , while the other has broader specificity An01g A similar pattern was observed for a well-expressed glucose transporter from cluster D An18g , coinciding with the disappearance of hexoses between 12 and 24 h.
Their continued expression at the later time points may be due to traces of hexoses or a delayed shut-down mechanism of their expression. Expression of the high-affinity hexose transporter encoding gene mstA was high at all time points 22 , but increased expression was observed over time, which fits with the reducing D-glucose concentrations at the later time points of the cultures.
A second high-affinity glucose transporter encoding gene, mstF 22 , was only highly expressed from 18 h onwards, while the low-affinity glucose transporter encoding mstC 22 was only highly expressed at 12 h, both providing correlation between affinity and residual D-glucose concentration. Surprisingly, the latter profile was also observed for mstG and mstH, which were reported to encode high-affinity glucose transporters However, the Km of these two transporters for glucose 0.
Some other transporters without experimental proof of function were highly expressed in the cultures, suggesting a role for them in the observed uptake Fig.
A putative pentose transporter of cluster B xatA was expressed during all time points, which would fit with a role in transport of both D-xylose and L-arabinose. Several putative galacturonic acid transporters were mainly expressed at the latter two time points, which coincides with the uptake of this compound. Of the three A. Expression of xltC was substantially induced at the first time point supporting its broad specificity for different sugars, while upregulation of xltB over time supported its relevance under environmental conditions with low availability of carbon sources The D-galacturonic acid transporter encoding gene gatA 25 was highly expressed at the first two time points, matching with the onset of D-galacturonic acid uptake, but downregulated after that.
In contrast with the results of a previous study 25 , the regulation of D-galacturonic acid catabolic genes was not connected to the uptake of D-galacturonic acid, as these genes were in fact downregulated during D-galacturonic acid uptake.
Getting Started with the Kaufmann Diet
This could be due to the higher expression of the gene encoding GaaX, the repressor of the D-galacturonic acid pathway, than the gene encoding GaaR, the activator of this pathway Overall, since most A. However, expression profiles of several known transporters match the sugar uptake profile.
Therefore, the unknown transporters with distinct expression patterns in our study would be highly interesting targets for functional analysis, e. Temporal uptake of sugars is not reflected in temporal catabolism profiles Surprisingly, the sequential uptake of sugars was not reflected in the catabolism of the individual sugar monomers, as the genes of the pathway related to a specific sugar are not specifically expressed when that sugar is taken up.
In contrast, D-galacturonic acid uptake occurs after 18 h, while the highest expression of this pathway is at 12 h Supplementary Fig.
Therefore, the physiology of A. The cluster analysis of the sugar catabolic gene expression data showed that the earliest time point 12 h differed most from the three later time points Fig.
Except for a general up-regulation of many of the catabolic genes at 12 h, there was no specific temporal expression profile for the genes representing any complete sugar catabolic pathway, as indicated in more detail by the individual pathway figures Supplementary Fig. For instance, only three genes of the pentose catabolic pathway show an increasing profile from 18 to 33 h Supplementary Fig.
S3D , and the same is observed for part of the Leloir pathway genes Supplementary Fig. Similarly, only two of the three L-rhamnose catabolic genes have a strong increase in expression from 24 to 33 h Supplementary Fig. Lemons and limes are allowed in phase 1. These fungi can also produce mycotoxins, or toxic by-products that can negatively impact your health and well being. Avoid the effects of harmful fungi in your body by adhering to Kaufman's Anti-Fungal Diet.
This diet "starves" the invading fungi, which gradually die off.
The Candida Diet: Beginner's Guide and Meal Plan
After the fungi die off, your body begins to heal from the damage the fungi inflicted in your body and you'll experience better health, writes Kaufman on LoveforLife. During phase 1, you eliminate all sugar, artificial sweeteners, grains, breaded meats, coffee and tea from your diet.
You also abstain from consuming pistachios, peanuts and peanut-containing products, green olives, salad dressings and yeast-containing products. Avoid potatoes, legumes, yams or butter-substitute products, such as margarine. Allowed Foods You can eat eggs, poultry, seafood and meat, yogurt, butter and sour cream.
Acceptable phase 1 foods include filtered or bottled water, lemonade sweetened with stevia, a natural, plant-based sweetener, avocados, berries, green apples, limes and lemons. You can also consume flax seed, olive and grape seed oils, raw nuts and seeds and unpasteurized apple cider vinegar.This is somewhat surprising, due to several reports of individual sugar transporters being under CreA control 17 , 18 , Therefore, the unknown transporters with distinct expression patterns in our study would be highly interesting targets for functional analysis, e.
The probe intensities were normalized for background by the robust multiarray average RMA method which makes use of only the perfect match probes. Organic chemists have attempted to classify them by their chemical structures e. Many efforts to address the mycotoxin problem simply involve the diversion of mycotoxin-contaminated commodities from the food supply through government screening and regulation programs.
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