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An informal panel discussion with the presenters will follow the presentations. Presenters will be available for an informal panel discussion in the final part of the workshop. In order to establish a solid ground, the workshop makes an attempt to come up with a widely acceptable definition and associated method of determining it! Furthermore, we are planning to discuss the limitations on spatial resolution and the associated relationship between sensitivity and resolution in case of commercially available techniques.
Metal Cationization of Biomolecules and its Analytical Applications Metal Ion Coordination Chemistry Interest Group Benjamin Bythell and Alex Shvartsburg presiding Room Mass spectrometry has been revolutionized since the s by the invention of soft sources such as electrospray ionization ESI and matrix-assisted laser desorption ionization MALDI that enabled intact ionization of increasingly large macromolecules. While ionization via attachment or withdrawal of one or more protons has been typical, addition of other charged groups such as metal cations is equally possible.
In particular, electron-transfer dissociation and similar direct mechanisms may fragment biomolecules cationized by a multiply-charged metal in a different manner than their polyprotonated analogs of same total charge. This workshop will encourage the discussion and adoption of novel analytical strategies that leverage metal cationization as an alternative to protonation in biological mass spectrometry.
The chair and co-chair will provide an interactive workshop with insights from experience while engaging the audience. Performance tracking is a key component of transferable science which is strengthened through quality metrics.
Confidence in results both within and across experiments lends itself to further application of discoveries to current and future work. DDA , metrics monitored fundamental ID free vs. ID metrics , frequency of evaluation, and type of standard employed simple vs.
Challenges and Progress towards the Site-Specific Characterization of Glycoprotein Heterogeneity Ron Orlando presiding Room An early step typically employed in the characterization of glycoprotein glycans involves the liberation of the glycans from the peptide backbone. While this process facilitates the characterization of the glycans, information on the glycan distribution at each site is lost.
The use of targeted SRM approaches will also be presented and discussed, as these allow site-specific heterogeneity to be determined in complex mixtures.
Methods that permit isomeric structural determination, such as MSn, will also be discussed. Mass Spectrometry Applications in Art, Cultural Heritage, and Natural History Mehdi Moini presiding Room The purpose of this workshop is to discuss the application of mass spectrometry MS to art and cultural heritage objects, as well as natural history specimens. This will be an interactive workshop in which various subjects relevant to the application of MS to art and natural history specimens will be discussed in a casual, dialog format.
A preliminary list of topics include: 1 Analysis of proteinaceous and organic specimens such as silk and wool textiles, leather and animal guts objects, bone and tissues, ink, paper, paint, coatings, binders, and wood. This DMPK-IG workshop will explore combining mass spectrometer improvements with microsampling of plasma, tissues and miniaturized assays to create better workflows to increase the quality of the DMPK data, and advance drug discovery and development projects.
In addition, the opportunities for obtaining early tissue distribution data from low amounts of tissue either by homogenization or microdialysis in discovery vs. This workshop will explore the opportunities and barriers of leveraging the full abilities of our modern mass spectrometers to take advantage of limited sample amounts.
Emerging tools to facilitate metabolomics research and new technologies will be discussed. The moderators will highlight recent developments in the field and survey the audience for their opinions.
A panel of invited scientists with expertise in field will be available to answer questions posed by the moderators and attendees. The workshop will close with a discussion where attendees can ask questions of the panelists. Some of the topics addressed will be: i new software tools for post-processing of untargeted metabolomics data, ii innovative experimental designs iii shotgun approaches with ion mobility, and iv metabolite identification by in silico fragmentation.
Which Career Path is Right for Me?
Topics will be focused on career planning and management, fundamental training, industrial internship, job search tools and interview strategies.
The panel, consisting of representatives from government, industrial and academic organizations, will share their knowledge and practices on career prospects. There are light refreshments in common areas. The guidance requirements will impact all aspects of mass spectrometric testing in the clinic, from therapeutic drug monitoring to companion diagnostics.
Additionally, the proposed guidance will affect the evolution of new biomarkers and new testing, particularly that of multi-index analyte tests.
Representatives from manufacturing, industry, regulatory bodies and advocacy groups will deliver brief presentations on their considerations of the proposed directives, followed by an open forum in which the expectations for the industry to deliver on the submission of analytical platforms and assays to the agency will be discussed.
The forum will provide a platform to share common themes, issues on these fronts and perhaps to surface newer needs in software, mass spec design, and automation. We sent out a survey with the participants prior to ASMS and the summary of the survey results were presented.
We had about attendees. Panel discussion with industry experts and thought leaders with the audience engagement was valuable. There was interest in continuing this workshop for In this workshop, we would like to present recent and future developments ProteomicsDB, a free, professionally developed solution to store and analyze mass spectrometry-based proteomics data.
ProteomicsDB has a strong focus on functionality and secondary use of proteomics and mass spectrometry data. Following up on a successful workshop at ASMS , we would like to encourage the involvement from the ASMS community, demonstrate typical use-cases for the web interface and API and describe our short and long-term plans.
The workshop will be open for discussion on applications, instrumentation, method development, and data analysis for high resolution tandem MS.
Tandem mass spectra from lipids and carbohydrates, on the other hand, have enjoyed considerably less bioinformatics support.
In this panel, the Bioinformatics Interest Group features an introduction to these classes of data from two researchers who have recently published algorithms to automate identification. Haixu Tang will discuss his efforts to recognize the structures of glycans and glycopeptides.
After a short informal presentation, less than ten minutes, the majority of the workshop would include an audience driven discussion with the opportunity to ask questions to a panel of experts. The organizers will have backup questions prepared for the panel to start or prompt the discussion if needed. The short presentation will provide an update on current workflows for ADC MS analysis and discuss details of the large range of characterization required for ADCs from initial MAb assessment to bioanalytical assay development.
To identify potential panelists, gauge the level of interest of the ASMS community, and tailor the discussion we will send out a survey of open ended questions in April.
Information Technology - New Generations
These data suggest that the majority of causative factors for chronic human disease is not genetic but rather exposures or some combination of exposures and the genome G. The exposome E is defined as the lifetime sum of these external and internal exposures.
Moreover, the exposome seeks the causative factors of disease to mitigate and prevent disease from occurring. The exposome is therefore a quantity of critical interest if we are to discover the non-genetic causative factors of chronic human diseases in a comprehensive manner. The exposome paradigm will facilitate the translation of applied research into educational, behavioral and policy-based, risk mitigating interventions. Advancements and Discussion of Mass Spectrometry Technology and Challenges within the Polymer and Material Fields Polymer and Material MS Interest Group Stephen Rumbelow and Gyorgy Vas presiding Room This workshop will focus on updating the group on recent work and challenges faced in the various fields such as academic, government, and industry.
The focus of this group is polymer and material analysis utilizing various mass spectrometric techniques for both characterization and quantitation of oligomeric species.
This workshop will explore the various ways that polymers and materials are not only analyzed themselves but also how they interact with other materials such as patients, and different type of products such as packaging and medical devices.
This workshop will cover the nutsand-bolts of the publication workflow with an emphasis on the peer review process. A panel of Editors will provide an inside look at how manuscripts are handled after submission, how reviewers are selected, and the role of the both authors and reviewers in the process.
Tips for being a top reviewer will be covered, as well as how to become involved as a new reviewer. Attendees will be divided into small groups for break-out discussions on topics such as career transitions, work-life balance and mentoring. Each small group will have an experienced scientist and facilitator. All are welcome. Attendees should bring business cards for distribution if possible. Often this challenging aspect is overlooked and under appreciated. Collaborators often have only a cursory understanding of what you did and communicating what may be wrong with the data you generated can be daunting.
With all of these different variations, many people have found confusion as to when to apply each technology. This year, we propose to continue where we left off last year and focus on the role that high resolution mass spectrometry will play in the regulatory environment. Eccentric Genius, Reluctant Prophet. Nuttala and a foreword to the edition by Jane Hipolito. Five keywords should be listed at the end of the abstract. Proofs First proof will be sent to the lead author or a nominee , who will be expected to return corrections to the Editor by e-mail within three days of receipt.
Submission of an article is taken to imply that it has not previously been published or is not being considered for publication elsewhere. If an author is publishing a related article elsewhere, this fact should be stated. No free offprints. The second part of this paper deals with the remarkably changed situation after the emergence of a new regime in Keywords: C.
Lewis, reception history, C. The responsibility for the final form of the text is entirely mine.
Several intellectuals, e. Chesterton and C. Later on, in the s and s, some of the few Romanians who had the opportunity to travel abroad enthusiastically read C. Lewis and played an important part in promoting his writings when the totalitarian system, marked by restrictions and censorship enforced by severe surveillance, collapsed.
In the present study I endeavour to retrace the main stages C. One fact needs to be specified from the very beginning: the author in question was an active member of the Anglican Church.
Obviously his fiction The Space Trilogy, The Chronicles of Narnia addresses an audience for whom his confessional membership would be of no interest.
We could say that Lewis permeated Romanian culture in four different ways, on four clearly detectable editorial paths. Of course, like every intellectual construct, the classification I propose is marked by a certain procrustean artificiality. In order to construct an intelligible not to say, intelligent outline, the explorer will inevitably need to segment reality into categories which are not always rigidly distinct.
An observation which should be made from the outset is that, at the moment, Lewis the scholar is missing from the Romanian book market altogether. The only translation that gives an inkling of C.
The book highlights his interest in literary criticism, but reveals nothing of his special competence as a medieval and renaissance scholar. The book was translated and printed abroad and then smuggled into Romania, where it was spread by underground means. The year when the book was released cannot be established with certainty, but those who had access to it during the Communist years testify that it was already available in In the following sections we will chronologically present the first three ways in which Romanian readers became acquainted with C.
Lewis Aqua Forte, Steinhardt, an Orthodox monk who converted to Christianity while serving a prison term under Communism. His diary reveals that he visited London between and that, during those years, he attended a few religious meetings of the Oxford Group Steinhardt , , Given that Steinhardt was well acquainted with G.
I believe C. The mortal would like to have his tea at 5 P. His wife and his mother-in-law prefer to have it inside. In the example given by the Romanian author, the husband who gives in to the preferences of his wife and mother-in-law is the victim of the temptation. LEWIS cheap generosity derived from petty motivations. Old resentments brought to bear in such a trivial conflict eventually lead to a disastrous situation. The difference in perspective between the Lewisian text and the interpretation given by Steinhardt seems to indicate that, at the moment when he was writing his Diary, Steinhardt did not have a fresh reading of The Screwtape Letters in mind.
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Besides, this is the only reference to Lewis in The Diary of Happiness. A more thorough research of the subject should also give due consideration to the books that are known to have belonged to the Romanian monk and which are now part of the library of the Rohia monastery. One would not be surprised to find that there are other works of C.
Lewis among the books of N. Due to the role he played in the attempt to oust the Groza government, he was arrested in and 1 Interview with A.
He found them intelligent, witty, and, most important, trans- denominational. Lewis impressed him because of the rigour he put into his writing. Avoiding doctrinaire positions, Lewis managed to go beyond the narrow dogmatic confines of any particular denomination and engage believers across all denominations. Lewis was to become instrumental for their diffusion in post Romanian society. He had settled in the USA in and is currently a professor of literature and philosophy at the Catholic University of America, where he has been teaching since Lewis Nemoianu It is a good general presentation of Lewis, with an emphasis on his conversion described in Surprised by Joy and his fiction especially the Narnia novels and the space trilogy.
The informed reader of the material Nemoianu broadcast will probably agree with his general view but not with some of the details. Between the two, there was a space of about two years during which Lewis tried to live a religious life in the absence of hope for a future life. In fact, C. Lewis expressed his political views on numerous occasions, and we need not infer his attitude towards Nazism or Communism by resorting to extraneous studies, since we are able to quote him directly.
As for the idea of Equality, Lewis spoke often about it. It is a result of the Fall and the remedy for it. Any attempt to retrace the steps by which we have arrived at egalitarianism and to re-introduce the old authorities on the political level is for me as foolish as it would be to take off our clothes. The Nazi and the Nudist make the same mistake.
But it is the naked body, still there beneath the clothes of each one of us, which really lives. Such a character would accept help from Fascists, convinced that he and his friends are the leaven which could influence positively the dough of their party.
Furthermore, he is tempted to accept help from the defenders of the capitalist status quo, whose commercial and imperial agenda does not retain even the least appearance of theism. Starting off from the premise that a radical change is needed, Spartacus demands a leftist revolution and is willing to accept help from those on the left who openly declare themselves enemies of God Lewis, God The readers will have realized by now that the three names represent three different ideologies: Fascism, Conservatism and Communism.
Following in J. That invaluable man Rousseau first revealed it. From that starting point, via Hegel another indispensable propagandist on our side , we easily contrived both the Nazi and the Communist state. Even in England we were pretty successful. I heard the other day that in that country a man could not, without a permit, cut down his own tree with his own axe, make it into planks with his own saw, and use the planks to build a tool shed in his own garden.
On the contrary, by virtue of his ethical and political acuity, he often publicly drew attention to the fallacies and delusions which can accompany the subtle semantic mutations constantly emerging in discourse on education, freedom, politics, values, and so on.
The edition contains no information about the date or place of publication, but the terminus 1 C. Hooper, New York, Macmillan, , p. The available information implies that the book was translated and distributed by a Romanian Baptist organization based in the USA. The translation was made by Livius Percy and edited by John Tipei. According to more plausible information, the initiative for translating and publishing the book came from a group of Baptist Christians in the United States, members of congregation in Chicago pastored by Petru Popovici.
Such books, printed in runs of about 3, copies, did not usually include all the customary bibliographic information —except for the printing house and year of publication. This was to protect individuals from retaliation by the Communist government. Once printed, the books were distributed to various organizations specializing in the clandestine dissemination of Christian literature within the Communist bloc. The version prepared for print before proved impossible to recover, so the initial handwritten draft of the translation was carefully reviewed with the help of Marinela Blaj, a collaborator of the publisher at the time.
I specialize in the diabolic. Not surprisingly, when Screwtape was published by Humanitas in , the interest in the old Logos version revived significantly. The low interest of the Evangelical market for C. However, the financial difficulties they encountered in recent years have left the project in an uncertain state.
Any analysis of the reception of C.Has Photoionization Reached its Potential? Polynomials with two given zeros. II, no. Similarly, we may define a "circular region" in E by an inequality H x, x? The term ; - zk -1 may be regarded as the force upon the unit mass at zk due to the unit mass at the variable point z,. As is well known, eqs. Thus C may be interpreted as the point at which all the mass must be concentrat in order to produce at Z the same resultant force as the system of masses m; at the points z;.
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